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FiberCheck PRO Software - download Your VIDA custom product will ship within 4-8 business days and will arrive at your starting address within 7-14 business days.. Learn more. Discover the pain point that FiberChekPRO helps with while.MMP-13 and TIMP-1 gene expression in benign and malignant thyroid tissues: an in vitro study. Matrix metalloproteinases (MMPs) are a family of proteolytic enzymes involved in a wide range of physiologic and pathologic processes, including cancer progression and metastasis. The regulation of their expression and activity is controlled by the interplay between active MMPs and their natural inhibitors called tissue inhibitors of metalloproteinases (TIMPs). Recently, a down-regulation of MMP-13 and TIMP-1 genes was observed in malignant thyroid cells, suggesting that altered MMP-13/TIMP-1 expression levels could be involved in tumor progression. The present work aimed at investigating MMP-13 and TIMP-1 gene expression in a model of human thyroid cell line, Nthy-ori-3-1, in vitro. The stable expression of the sense or antisense of MMP-13, TIMP-1, and control genes was obtained in Nthy-ori-3-1 cells, and the levels of expression of MMP-13 and TIMP-1 were detected by reverse transcription polymerase chain reaction (RT-PCR). Cell growth was studied, and by using the cell cycle assay, apoptosis rate, caspase-3 activity, and growth inhibitory factor (GIF) was studied. The results indicate that the expression of both MMP-13 and TIMP-1 was increased when cells were stably transfected with the sense MMP-13, whereas both MMP-13 and TIMP-1 expression were decreased when cells were stably transfected with the antisense MMP-13. The sense and antisense of MMP-13, TIMP-1, and control genes were more toxic for Nthy-ori-3-1 cells, showing that the transfection with antisense TIMP-1 enhanced caspase-3 activity, apoptosis rate, and GIF expression and cell growth inhibition. Therefore, the results demonstrate that MMP-13 and TIMP-1 expression is related to the progression of thyroid tumors, suggesting that the decreased expression of these genes could be involved in the progression of









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Fiber Check Pro Software Download pervan

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